Reactivity: | H,M,R & M,R |
Applications: | WB & IHC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | GTF2E2 rabbit polyclonal antibody |
Gene Name: | General transcription factor IIE subunit 2 |
Synonyms: | Transcription initiation factor IIE subunit beta, TFIIE-beta, Gtf2e2, FE, TF2E2 |
Immunogen: | Recombinant protein corresponding to Mouse GTF2E2 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. | 33 kDa |
Observed MW. | 33 kDa |
Uniprot ID: | P29084, Q9D902 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 1000-1: 3000 | heart, brain, testis |
IHC/IF | Mouse, Rat | 1: 600-1: 6000 | heart, liver, testis |
Background
Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.
Images
Western blot analysis of TF2E2 (GB112155) at dilution of 1: 1000 | |
Immunohistochemistry of paraffin embedded mouse liver using TF2E2 (GB112155) at dilution of 1: 3000 (300x lens) | |
Immunohistochemistry of paraffin embedded mouse testis using TF2E2 (GB112155) at dilution of 1: 3000 (350x lens) | |
Immunohistochemistry of paraffin embedded rat liver using TF2E2 (GB112155) at dilution of 1: 3000 (300x lens) | |
Immunohistochemistry of paraffin embedded rat testis using TF2E2 (GB112155) at dilution of 1: 3000 (260x lens) | |
Immunofluorescence of paraffin embedded mouse heart using TF2E2 (GB112155) at dilution of 1: 600 (400x lens) | |
Immunofluorescence of paraffin embedded mouse testis using TF2E2 (GB112155) at dilution of 1: 600 (260x lens) | |
Immunofluorescence of paraffin embedded rat liver using TF2E2 (GB112155) at dilution of 1: 600 (400x lens) | |
Immunofluorescence of paraffin embedded rat testis using TF2E2 (GB112155) at dilution of 1: 600 (260x lens) |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |