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Recombinant Anti-Glutamine Synthetase Mouse mAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Glutamine synthetase |
Synonyms: | cell proliferation-inducing protein 59, GLNA, GLNS, GLUL, Glutamate ammonia ligase, Glutamate decarboxylase, Glutamine synthetase, GS, PIG43, PIG59, Proliferation inducing protein 43, Palmitoyltransferase GLUL |
Immunogen: | Recombinant protein corresponding to Human Glutamine Synthetase |
Uniprot ID: | P15104 |
Isotype: | IgG1 |
Purity: | Affinity purification |
Subcellular location: | Cell membrane, Cytoplasm |
Predicted MW. / Observed MW. | 42 kDa / 42 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | liver, brain |
IHC/IF | Human, Mouse, Rat | 1: 3000-1: 6000 | glioblastoma, Inflammation of the liver |
Background
Glutamine Synthetase catalyzes the conversion of ammonia and glutamate to glutamine. It is found in astrocytes as an octamer of identical 42 kDa subunits. The function of Glutamine Synthetase is the detoxification of brain ammonia. It also has an important role in the metabolic regulation of neurotransmitter glutamate. Because of the multiple functions and importance of Glutamine Synthetase in cellular metabolism, both catalytic activities and synthesis are highly regulated. The activity of Glutamine Synthetase is controlled by adenylylation.
Images
| WB analysis of Glutamine Synthetase（GB151177). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h. |
| WB analysis of Glutamine Synthetase（GB151177). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h. |
| IHC analysis of Glutamine Synthetase(GB151177). Sample: Human brain, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203)), 98°C, 20 min. Primary antibody: 1: 5000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min. |
| IHC analysis of Glutamine Synthetase(GB151177). Sample: Human glioblastoma, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203)), 98°C, 20 min. Primary antibody: 1: 5000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min. |
| IHC analysis of Glutamine Synthetase(GB151177). Sample: Human liver, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203)), 98°C, 20 min. Primary antibody: 1: 5000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min. |
| IHC analysis of Glutamine Synthetase(GB151177). Sample: Mouse Inflammation of the liver, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203)), 98°C, 20 min. Primary antibody: 1: 5000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min. |
| IHC analysis of Glutamine Synthetase(GB151177). Sample: Rat brain, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203)), 98°C, 20 min. Primary antibody: 1: 5000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min. |
| IHC analysis of Glutamine Synthetase(GB151177). Sample: Rat liver, 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203)), 98°C, 20 min. Primary antibody: 1: 5000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L), Ready to use(G1301), RT, 20min. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
