Recombinant Anti-Glutathione Peroxidase 4 Mouse mAb (100 μl)

Phospholipid hydroperoxide glutathione peroxidase

Synonyms:

PHGPx, Glutathione peroxidase 4, GPx-4, GSHPx-4, Gpx4, snGPx, snPHGPx

Immunogen:

Recombinant protein corresponding to Mouse GPX4

Uniprot ID:

O70325

Isotype:

IgG1

Purity:

Affinity purification

Subcellular location:

Cytoplasm, Nucleus

Predicted MW. / Observed MW.

22 kDa / 20-23 kDa

WB

Human, Mouse, Rat

1: 500-1: 1000

brain, testis

IHC/IF

Mouse, Rat

1: 500-1: 2000

testis

WB analysis of Glutathione Peroxidase 4(GB154327).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h.

IHC analysis of Glutathione Peroxidase 4(GB154327).
Sample: Mouse testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.

IHC analysis of Glutathione Peroxidase 4(GB154327).
Sample: Rat testis, 4% PFA (G1101) 12-24h.
Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.