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Anti-Grp75/MOT Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Grp75/MOT rabbit polyclonal antibody |
Gene Name: | Stress-70 protein, mitochondrial |
Synonyms: | 75 kDa glucose-regulated protein, GRP-75, Heat shock 70 kDa protein 9, Mortalin, Peptide-binding protein 74, PBP74, p66 MOT, Hspa9, rp75, Hsp74, Hspa9a |
Immunogen: | Recombinant protein corresponding to Mouse Grp75/MOT |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Mitochondrion, Nucleus |
Predicted MW. | 73 kDa |
Observed MW. | 73 kDa |
Uniprot ID: | P38646, P38647, P48721 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | kidney, colon |
IHC/IF | Human, Mouse, Rat | 1: 600-1: 6000 | brain, colon, colon cancer, kidney, liver cancer, ovarian cancer |
Background
This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.
Images
| Western blot analysis of Grp75 (GB112273) at dilution of 1: 500 |
| Immunohistochemistry of paraffin embedded human colon cancer using GRP75 (GB112273) at dilution of 1: 3000 (400x lens) |
| Immunohistochemistry of paraffin embedded human liver cancer using GRP75 (GB112273) at dilution of 1: 3000 (400x lens) |
| Immunohistochemistry of paraffin embedded human ovarian cancer using GRP75 (GB112273) at dilution of 1: 3000 (400x lens) |
| Immunofluorescence of paraffin embedded mouse brain using GRP75 (GB112273) at dilution of 1: 650 (400x lens) |
| Immunofluorescence of paraffin embedded rat colon using GRP75 (GB112273) at dilution of 1: 650 (400x lens) |
| Immunofluorescence of paraffin embedded rat kidney using GRP75 (GB112273) at dilution of 1: 650 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
