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| Reactivity: | H |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | HEXO rabbit polyclonal antibody |
Gene Name: | 3'-5' exoribonuclease 1 |
Synonyms: | 3'-5' exonuclease ERI1, Eri-1 homolog, Histone mRNA 3'-exonuclease 1, 3'exo, Thex1, Eri1, Protein 3''hExo |
Immunogen: | Recombinantproteincorrespondingto Mouse HEXO |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 39 kDa |
Observed MW. | 46 kDa |
Uniprot ID: | Q8IV48 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human | 1: 500-1: 1000 | K562, HepG2 |
Background
RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.
Images
| Western blot analysis of ERI1 (GB111698) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
