Reactivity: | H |
Applications: | WB |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | Hexokinase Type III/HK3 rabbit polyclonal antibody |
Gene Name: | Hexokinase-3 |
Synonyms: | Hexokinase type III, HK III, Hexokinase-C, Hk3, ATP:D hexose 6 phosphotransferase |
Immunogen: | Recombinant protein corresponding to Mouse Hexokinase Type III/HK3 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 100 kDa |
Observed MW. | 100 kDa |
Uniprot ID: | P52790 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human | 1: 500-1: 1000 | HeLa, THP-1, HepG2, NCI-H460, A549 |
Background
Hexokinases phosphorylate glucose to produce glucose 6 phosphate, thus committing glucose to the glycolytic pathway. In vertebrates there are four major glucose phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase). Similar to hexokinases I and II, the allosteric enzyme hexokinase III is inhibited by its product glucose 6 phosphate. The catalytic activity of hexokinase III is associated with the C terminus while regulatory function is associated with the N terminus.
Images
Western blot analysis of Hexokinase3 (GB111717) at dilution of 1: 500 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |