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Anti-Histone H2A.X Rabbit pAb (100 μl)
| Reactivity: | H,M,R & M,R & H,M,R |
| Applications: | WB & IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Histone H2A.X rabbit polyclonal |
Gene Name: | Histone H2AX |
Synonyms: | H2A histone family, member X, H2A.X, H2A/X, H2AFX,H2AX, Histone H2A.x |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human Histone H2A.X |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | |
Predicted MW. | 15 kDa |
Observed MW. | 15 kDa |
Uniprot ID: | P16104, P27661 |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | testis, kidney, thymus |
IHC | Mouse, Rat | 1: 1600-1: 3200 | testis, kidney |
IF | Mouse, Rat | 1: 100-1: 500 | kidney, testis |
Background
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.
Images
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Western blot analysis of H2AX (GB111560) at dilution of 1: 1000 |
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Immunohistochemistry analysis of paraffin-embedded mouse testis using H2AX (GB111560) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded mouse testis using H2AX (GB111560) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded mouse kidney using H2AX (GB111560) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded rat kidney using H2AX (GB111560) at dilution of 1: 2000 |
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Immunofluorescent analysis of paraformaldehyde-fixed mouse kidney using H2AX (GB111560) at dilution of 1: 300 |
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Immunofluorescent analysis of paraformaldehyde-fixed rat kidney using H2AX (GB111560) at dilution of 1: 300 |
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Immunofluorescent analysis of paraformaldehyde-fixed mouse testis using H2AX (GB111560) at dilution of 1: 300 |
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Immunofluorescent analysis of paraformaldehyde-fixed rat testis using H2AX (GB111560) at dilution of 1: 300 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed HeLa cells using H2AX(GB111560) at dilution of 1:100-1:400 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
