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Anti-Histone H2A.X Rabbit pAb (100 μl)
| Reactivity: | M |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Histone H2AX |
Synonyms: | H2A histone family, member X, H2A.X, H2A/X, H2AFX, H2AX, Histone H2A.x |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Histone H2A.X |
Uniprot ID: | P27661 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Chromosome |
Predicted MW. / Observed MW. | 15 kDa / 15 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | kidney, liver, colon |
IHC/IF | Mouse, Rat | 1: 100-1: 1000 | testis |
Background
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Images
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Western blot analysis of Histone H2AX (GB11365) at dilution of 1: 1000 |
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Immunohistochemistry analysis of paraffin-embedded mouse splenitis using Histone H2A.X (GB11365 at dilution of 1: 1500 |
 | Immunohistochemistry analysis of paraffin-embedded mouse testis using Histone H2A.X (GB11365 at dilution of 1: 1500 |
 | Immunohistochemistry analysis of paraffin-embedded rat testis using Histone H2A.X (GB11365 at dilution of 1: 1500 |
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Immunofluorescent analysis of paraffin-embedded mouse testis using Histone H2A.X (GB11365 at dilution of 1: 1500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
