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Anti-Histone H4 Rabbit pAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Histone H4 rabbit polyclonal antibody |
Gene Name: | Histone H4 |
Synonyms: | H4F2, H4FN, HIST1H4, HIST2H4, HISTH4H4, H4c1, Hist1h4a, H4c2, H4-53, Hist1h4b, H4c3, H4-12, Hist1h4c, H4c4, Hist1h4d, Hist1h4f, H4c6, H4c14, Hist2h4, Hist2h4a |
Immunogen: | Recombinant protein corresponding to Human Histone H4 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Uniprot ID: | P62805, P62806 |
Product Usage Information
IHC/IF | Human | 1: 1000-1: 2000 | adenoma, breast cancer, colon |
ICC/IF | Human | 1: 50-1: 300 | HeLa |
Background
Histone H4 H4 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H4 is involved with the structure of the nucleosome of the 'beads on a string' organization. Histone proteins are highly post-translationally modified. Covalently bonded modifications includeacetylation and methylation of the N-terminal tails. These modifications may alter expression of genes located on DNA associated with its parent histone octamer.
Images
 | Immunohistochemistry of paraffin embedded human adenoma using Histone H4 (GB11508) at dilution of 1:1000 (400x lens) |
 | Immunohistochemistry of paraffin embedded human colon using Histone H4 (GB11508) at dilution of 1:1000 (400x lens) |
 | Immunohistochemistry of paraffin embedded human lung using Histone H4 (GB11508) at dilution of 1:1000 (400x lens) |
| Immunofluorescence of paraffin embedded human breast cancer using Histone H4 (GB11508) at dilution of 1:1000 (400x lens) |
| Immunofluorescence of paraffin embedded human uterus cancer using Histone H4 (GB11508) at dilution of 1:1000 (400x lens) |
| ICC analysis of Histone H4(GB11508)red. Sample: HeLa, 4% PFA (G1101) 20min. Primary antibody: 1: 50, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
