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Recombinant Anti-IKB alpha Mouse mAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | NF-kappa-B inhibitor alpha |
Synonyms: | I kappa B alpha, IkappaBalpha, IkB alpha, IKBA, Ikβ-α, MAD 3, MAD3, NF kappa B inhibitor alpha, NFKBI, NFKBIA |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human IKBα |
Uniprot ID: | P25963 |
Isotype: | IgG1 |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 36 kDa / 36 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | HeLa+Calyculin A,NIH/3T3+Calyculin A, HeLa, NIH3T3 |
Background
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
Images
| WB analysis of IKB alpha (GB151509). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1:1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles.|
| Storage Buffer | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
