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| Reactivity: | H |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Interleukin-8 ligand 8 |
Synonyms: | CXCL8, 3-10C, AMCF-I, b-ENAP, GCP-1, GCP1, IL-8, IL8, K60, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, SCYB8, TSG-1 |
Immunogen: | Recombinant protein corresponding to Human IL-8 |
Uniprot ID: | P10145 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 11 kDa / 11 kDa |
Product Usage Information
WB | Human | 1: 300-1: 600 | PC3, THP-1 |
Background
Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection . Also plays an important role in neutrophil activation . Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells . G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways .
Images
| Western blot analysis of IL-8 (GB115271) at dilution of 1: 2000 |
| Western blot analysis of IL-8 (GB115271) at dilution of 1: 300 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
