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| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | KAP1 rabbit polyclonal antibody |
Gene Name: | Transcription intermediary factor 1-beta |
Synonyms: | TIF1-beta, E3 SUMO-protein ligase TRIM28, KRAB-A-interacting protein, KRIP-1, RING-type E3 ubiquitin transferase TIF1-beta, Trim28, Kap1, Krip1, Tif1b |
Immunogen: | Recombinant protein corresponding to Mouse KAP1 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. | 54/89 kDa |
Observed MW. | 75/110 kDa |
Uniprot ID: | Q13263, Q62318, O08629 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | brain, spleen, thymus |
IHC/IF | Human, Mouse, Rat | 1: 400-1: 1200 | brain, testis |
Background
Nuclear corepressor for KRAB domain-containing zinc finger proteins (KRAB-ZFPs). Mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 (which specifically methylates histone H3 at 'Lys-9' (H3K9me)) to the promoter regions of KRAB target genes.
Images
| Western blot analysis of TRIM28 (GB112038) at dilution of 1: 500 |
| Immunohistochemistry of paraffin embedded human testis using TRIM28 (GB112038) at dilution of 1: 1200 (300x lens) |
| Immunohistochemistry of paraffin embedded mouse brain using TRIM28 (GB112038) at dilution of 1: 600 (200x lens) |
| Immunohistochemistry of paraffin embedded mouse testis using TRIM28 (GB112038) at dilution of 1: 1200 (250x lens) |
| Immunohistochemistry of paraffin embedded rat testis using TRIM28 (GB112038) at dilution of 1: 1200 (200x lens) |
| Immunofluorescence of paraffin embedded mouse testis using TRIM28 (GB112038) at dilution of 1: 650 (400x lens) |
| Immunofluorescence of paraffin embedded rat testis using TRIM28 (GB112038) at dilution of 1: 650 (250x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
