Reactivity: | M |
Applications: | IHC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Gene Name: | Lymphatic vessel endothelial hyaluronic acid receptor 1 |
Synonyms: | CRSBP-1, CRSBP1, HAR, Hyaluronic acid receptor, LYVE-1, LYVE1, XLKD1, Cell surface retention sequence-binding protein 1 |
Immunogen: | KLH conjugated Synthetic peptidecorresponding to Mouse LYVE1 |
Uniprot ID: | Q8BHC0 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell Membrane |
Product Usage Information
IHC/IF | Mouse | 1: 2000-1: 4000 | small intestine, lymph node |
Background
LYVE1 is a 322-amino acid type-I integral membrane glycoprotein primarily expressed on lymphatic endothelial cells. LYVE1 is a CD44 homologue. It acts as a receptor and binds to both soluble and immobilized hyaluronan. LYVE1 may function in lymphatic hyaluronan transport and have a role in tumor metastasis.
Images
IHC analysis of lyve-1 (GB113499). Sample: Mouse small intestine (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 2000, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. | |
IF analysis of C1005 (GB113499). Sample: Mouse lymph node (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98°C,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 3600, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |