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Anti-MANEA Rabbit pAb (100 μl)
| Reactivity: | H |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Glycoprotein endo-alpha-1,2-mannosidase |
Synonyms: | ENDO, Endo alpha mannosidase, Endomannosidase, hEndo, Mandaselin, MANEA, mannosidase, endo alpha |
Immunogen: | Recombinant protein corresponding to Mouse MANEA |
Uniprot ID: | Q6NXH2 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Golgi apparatus membrane |
Product Usage Information
IHC/IF | Human | 1: 1200-1: 2400 | tonsil, placenta |
Background
N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi.
Images
| Immunohistochemistry analysis of paraffin embedded human tonsil using MANEA (GB114171) at dilution of 1: 2400 |
| Immunohistochemistry analysis of paraffin embedded human placenta using MANEA (GB114171) at dilution of 1: 1200 |
| Immunofluorescence analysis of paraffin embedded human tonsil using MANEA (GB114171) at dilution of 1: 1200 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
