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Anti-MAPRE1/EB1 Rabbit pAb (100 μl)
| Reactivity: | H,M |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | EB1 rabbit polyclonal antibody |
Gene Name: | Microtubule-associated protein RP/EB family member 1 |
Synonyms: | APC-binding protein EB1, End-binding protein 1, EB1, Mapre1 |
Immunogen: | Recombinant protein corresponding to Mouse EB1 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 30 kDa |
Observed MW. | 35 kDa |
Uniprot ID: | Q61166 |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse | 1: 500-1: 1000 | colon, thymus, liver, kidney |
Background
The EB1 protein Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes cytoplasmic microtubule nucleation and elongation. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement. Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules. Acts as a regulator of autophagosome transport via interaction with CAMSAP2 (By similarity). May play a role in cell migration.
Images
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Western blot analysis of EB1 (GB111069) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
