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Anti-MBD4/MED1 Rabbit pAb (100 μl)
| Reactivity: | H,M & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Methyl-CpG-binding domain protein 4 |
Synonyms: | Methyl-CpG-binding protein MBD4, Mismatch-specific DNA N-glycosylase, MBD4, MED1, G/U mismatch glycosylase, MBD4/MED1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse MBD4 |
Uniprot ID: | Q9Z2D7 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. / Observed MW. | 63 kDa / 70 kDa |
Product Usage Information
WB | Human, Mouse | 1: 500-1: 1000 | brain |
IHC/IF | Human, Mouse, Rat | 1: 300-1: 2000 | testis, brain |
Background
MBD4, also named as MED1, is a 580 amino acid protein, which Interacts with MLH1. MBD4 as a mismatch-specific DNA N-glycosylase is involved in DNA repair and has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. MBD4 can also remove uracil or 5-fluorouracil in G:U mismatches. MBD4 exists some isoforms with MV 66 kDa and 30 kDa (The identification of a novel alternatively spliced form of the MBD4 DNA glycosylase).
Images
| Western blot analysis of MBD4 (GB111879) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded mouse testis using MBD4 (GB111879) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraffin embedded rat testis using MBD4 (GB111879) at dilution of 1: 300 |
| Immunofluorescent analysis of paraffin embedded mouse testis using MBD4 (GB111879) at dilution of 1: 300 |
| Immunofluorescent analysis of paraffin embedded mouse brain using MBD4 (GB111879) at dilution of 1: 300 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
