Reactivity: | H,M,R & H,M,R |
Applications: | WB & IHC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | MCM2 rabbit polyclonal antibody |
Gene Name: | DNA replication licensing factor MCM2 |
Synonyms: | Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, Mcm2, Bm28, Cdcl1, Kiaa0030, Mcmd2 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse MCM2 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. | 102 kDa |
Observed MW. | 130 kDa |
Uniprot ID: | P49736, P97310, D3ZP96 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | thymus |
IHC/IF | Human, Mouse, Rat | 1: 1000-1: 4000 | brain, colon cancer, lung, lung cancer, thymus |
Background
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex.
Images
Western blot analysis of MCM2 (GB111162) at dilution of 1: 500 |
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Immunohistochemistry of paraffin embedded human colon cancer using MCM2 (GB111162) at dilution of 1: 4000 (400x lens) |
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Immunohistochemistry of paraffin embedded human lung cancer using MCM2 (GB111162) at dilution of 1: 4000 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse brain using MCM2 (GB111162) at dilution of 1: 2000 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse thymus using MCM2 (GB111162) at dilution of 1: 4000 (400x lens) |
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Immunohistochemistry of paraffin embedded rat lung using MCM2 (GB111162) at dilution of 1: 2000 (400x lens) |
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Immunohistochemistry of paraffin embedded rat thymus using MCM2 (GB111162) at dilution of 1: 4000 (400x lens) |
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Immunofluorescence of paraffin embedded mouse brain using MCM2 (GB111162) at dilution of 1: 2000 (400x lens) |
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Immunofluorescence of paraffin embedded rat thymus using MCM2 (GB111162) at dilution of 1: 2000 (400x lens) |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |