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Anti-MED13/GCT Rabbit pAb (100 μl)
| Reactivity: | M & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | MED13/GCT rabbit polyclonal antibody |
Gene Name: | Mediator of RNA polymerase II transcription subunit 13 |
Synonyms: | Thyroid hormone receptor-associated protein 1, Thyroid hormone receptor-associated protein complex 240 kDa component, Trap240, Med13, Kiaa0593, Thrap1 |
Immunogen: | Recombinant protein corresponding to Mouse MED13/GCT |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. | 239 kDa |
Observed MW. | 250 kDa |
Uniprot ID: | Q5SWW4 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Mouse | 1: 250-1: 500 | skeletal muscle |
IHC/IF | Mouse, Rat | 1: 700-1: 1400 | corpus striatum, lung, substantia nigra |
Background
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Images
 | Western blot analysis of MED13 (GB112350) at dilution of 1: 250 |
 | Immunofluorescence of paraffin embedded mouse corpus striatum using MED13 (GB112350) at dilution of 1: 700 (300x lens) |
 | Immunofluorescence of paraffin embedded mouse lung using MED13 (GB112350) at dilution of 1: 700 (400x lens) |
 | Immunofluorescence of paraffin embedded rat lung using MED13 (GB112350) at dilution of 1: 700 (300x lens) |
 | Immunofluorescence of paraffin embedded rat substantia nigra using MED13 (GB112350) at dilution of 1: 700 (250x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
