Reactivity: | H,M,R & H,M |
Applications: | IHC/IF & ICC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Gene Name: | N-terminal Xaa-Pro-Lys N-methyltransferase 1 |
Synonyms: | Alpha N-terminal protein methyltransferase 1A, Methyltransferase-like protein 11A, Ntmt1, NTM1A, Mettl11a, X-Pro-Lys N-terminal protein methyltransferase 1A |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse METTL11A |
Uniprot ID: | Q9BV86, Q8R2U4, Q5BJX0 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Cytoplasm |
Images
IHC/IF | Human, Mouse, Rat | 1: 800-1: 2000 | ung cancer, lung, liver cancer, colon cancer, testis, spleen, kidney, ovary, brain |
ICC/IF | Human, Mouse | 1: 200-1: 800 | HeLa |
Background
The METTL11A gene encodes an N-terminal methyltransferase for the RAN guanine nucleotide exchange factor regulator of chromosome condensation 1 (RCC1). METTL11A enzyme alpha-N-methylates other protein targets such as SET and RB. Required during mitosis for normal bipolar spindle formation and chromosome segregation.
Images
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Mettl11a (GB111391) at dilution of 1: 1500 |
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Immunohistochemistry analysis of paraffin-embedded mouse lung using Mettl11a (GB111391) at dilution of 1: 1500 |
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Immunohistochemistry analysis of paraffin-embedded human liver cancer using Mettl11a (GB111391) at dilution of 1: 1500 |
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Immunohistochemistry analysis of paraffin-embedded human colon cancer using Mettl11a (GB111391) at dilution of 1: 1500 |
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Immunohistochemistry analysis of paraffin-embedded mouse testis using Mettl11a (GB111391) at dilution of 1: 1500 |
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Immunohistochemistry analysis of paraffin-embedded mouse spleen using Mettl11a (GB111391) at dilution of 1: 1500 |
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Immunohistochemistry analysis of paraffin-embedded mouse kidney using Mettl11a (GB111391) at dilution of 1: 1500 |
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Immunohistochemistry analysis of paraffin-embedded rat ovary using Mettl11a (GB111391) at dilution of 1: 1500 |
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Immunohistochemistry analysis of paraffin-embedded rat brain using Mettl11a (GB111391) at dilution of 1: 1500 |
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Immunofluorescent analysis of paraformaldehyde-fixed mouse tonsil using Mettl11a (GB111391) at dilution of 1: 1500 |
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Immunofluorescent analysis of paraformaldehyde-fixed mouse testis using Mettl11a (GB111391) at dilution of 1: 1500 |
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Immunofluorescent analysis of paraformaldehyde-fixed fetal mouse using Mettl11a (GB111391) at dilution of 1: 1500 |
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Immunofluorescent analysis of paraformaldehyde-fixed rat adrenal gland using Mettl11a (GB111391) at dilution of 1: 150 |
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Immunofluorescent analysis of paraformaldehyde-fixed rat eyeball using Mettl11a (GB111391) at dilution of 1: 1500 |
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ICC analysis of METTL11A(GB111391)red. Sample: HeLa, 4% PFA (G1101) 20min. Primary antibody: 1: 400, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |