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Anti-METTL3 Mouse mAb (100 μl)
| Reactivity: | H,M,R & H |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | N6-adenosine-methyltransferase subunit METTL3 |
Synonyms: | IME4, M6A, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8 |
Immunogen: | Recombinant protein corresponding to Human METTL3 |
Uniprot ID: | Q86U44 |
Isotype: | IgG2b,κ |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Cytoplasm |
Predicted MW. / Observed MW. | 64kDa / 70kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | testis, spleen, brain |
IHC/IF | Human | 1: 500-1: 1000 | colon, testis, duodenum, colon cancer, ovarian cancer, esophageal cancer |
Background
METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.
Images
| Western blot analysis of METTL3 (GB124688) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded mouse colon using METTL3 (GB124688) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded mouse testis using METTL3 (GB124688) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded rat testis using METTL3 (GB124688) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded rat duodenum using METTL3 (GB124688) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded human colon cancer using METTL3 (GB124688) at dilution of 1: 500 |
| Immunohistochemistry analysis of paraffin embedded human ovarian cancer using METTL3 (GB124688) at dilution of 1: 500 |
| Immunohistochemistry analysis of paraffin embedded human esophageal cancer using METTL3 (GB124688) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
