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Anti-MIC19 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & M |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | MICOS complex subunit Mic19 |
Synonyms: | CHCHD3, MIC19, MINOS3 |
Immunogen: | Recombinant protein corresponding to Mouse CHCHD3 |
Uniprot ID: | Q9CRB9 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 26 kDa / 26 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 3000-1: 5000 | liver, skeletal muscle, lung |
ICC/IF | Mouse | 1: 50-1: 200 | NIH-3T3 |
Background
Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane. Has also been shown to function as a transcription factor which binds to the BAG1 promoter and represses BAG1 transcription. Plays an important role in the maintenance of the MICOS complex stability and the mitochondrial cristae morphology.
Images
| WB analysis of MIC19(GB114366). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 3000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| ICC analysis of MIC19(GB114366)red. Sample: NIH-3T3, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
