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| Reactivity: | H |
| Applications: | WB |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | DNA mismatch repair protein Mlh1 |
Synonyms: | MutL protein homolog 1, COCA2, FCC2, hMLH1, HNPCC, HNPCC2, MLH1 |
Immunogen: | Recombinant protein corresponding to MLH1 |
Uniprot ID: | P40692 |
Isotype: | IgG1,κ |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 85 KDa / 85 KDa |
Product Usage Information
WB | Human | 1: 500-1: 1000 | HeLa,Jurkat,MCF7 |
Background
Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH3) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2.
Images
| WB analysis of MLH1 (GB121958). |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
