Anti-MLKL Rabbit pAb (100 μl)

Mixed lineage kinase domain-like protein

Synonyms:

hMLKL, Mixed lineage kinase domain like, Mixed lineage kinase domain like protein, Mixed lineage kinase domain-like protein, mixed lineage kinase domain like pseudokinase

Immunogen:

KLH conjugated Synthetic peptide corresponding to Human MLKL

Uniprot ID:

Q8NB16

Isotype:

IgG

Purity:

Affinity purification

Subcellular location:

Cytoplasm, Cell membrane, Nucleus

Predicted MW. / Observed MW.

54 kDa / 54 kDa

Applications

Species

Dilution

Positive Tissue

WB

Human

1: 500-1: 1000

HeLa, HT-29, PC-3

IHC/IF

Human

1: 500-1: 1000

liver cancer, tonsil

WB analysis of MLKL（GB115699).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h.

IF analysis of MLKL(GB115699) red.
Sample: Human liver cancer, 4% PFA (G1101) 12-24h.
Blocking buffer: Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.

IF analysis of MLKL(GB115699) red.
Sample: Human tonsil, 4% PFA (G1101) 12-24h.
Blocking buffer: Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h.