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Anti-MRPS12 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | 28S ribosomal protein S12, mitochondrial |
Synonyms: | MRP-S12, MT-RPS12, Mrps12, S12mt mitochondrial Rpms12, Mitochondrial ribosomal protein S12, RT12_HUMAN, Mrps12 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse MRPS12 |
Uniprot ID: | O35680 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 15 kDa / 15 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | heart, liver |
ICC/IF | Human, Mouse | 1: 50-1: 200 | HeLa, NIH-3T3 |
Background
MRPS12 encodes a 28S subunit protein that belongs to the ribosomal protein S12P family. The encoded protein is a key component of the ribosomal small subunit and controls the decoding fidelity and susceptibility to aminoglycoside antibiotics. The gene for mitochondrial seryl-tRNA synthetase is located upstream and adjacent to this gene, and both genes are possible candidates for the autosomal dominant deafness gene (DFNA4). Splice variants that differ in the 5' UTR have been found for this gene; all three variants encode the same protein.
Images
|
Western blot analysis of MRPS12 (GB111570) at dilution of 1: 500 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed HeLa cells using MRPS12(GB111570) at dilution of 1: 50-1: 200 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH-3T3 cells using MRPS12(GB111570) at dilution of 1: 50-1: 200 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
