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| Reactivity: | H,M |
| Applications: | IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Mucin-2 |
Synonyms: | MUC2, MLP, MUC-2, SMUC, mucin 2, oligomeric mucus/gel-forming, Intestinal mucin 2, MUC 2, Mucin 2 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human MUC2 |
Uniprot ID: | Q02817 |
Isotype: | IgG1,k |
Purity: | Affinity purification |
Subcellular location: | Secreted |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
IHC/IF | Human, Mouse | 1: 500-1: 1000 | colon |
Background
This gene encodes a member of the mucin protein family. The protein encoded by this gene, also called mucin 2, is secreted onto mucosal surfaces.Mucin 2 is particularly prominent in the gut where it is secreted from goblet cells in the epithelial lininginto the lumen of the large intestine. There, mucin 2, along with small amounts of related-mucin proteins,polymerizes into a gel of which 80% by weight is oligosaccharide side-chains that are added as post-translational modifications to the mucin proteins.
Images
| IHC analysis of MUC2（GB120002）. Sample: Human colon, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 3000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
| IHC analysis of MUC2（GB120002）. Sample: Mouse colon, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 3000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
