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Anti-non-muscle Myosin IIB/MYH10 Rabbit pAb (100 μl)
| Reactivity: | H,M & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | MYH10 rabbit polyclonal antibody |
Gene Name: | Myosin-10 |
Synonyms: | Cellular myosin heavy chain, type B, Myosin heavy chain 10, Myosin heavy chain, MYH10, non-muscle IIb, NMMHC-B, NMMHC-IIB |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse MYH10 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nuclear, Cytoplasm, Secreted |
Predicted MW. | 229 kDa |
Observed MW. | 260 kDa |
Uniprot ID: | P35580, Q61879, Q9JLT0 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse | 1: 500-1: 800 | brain, kidney |
IHC/IF | Mouse, Rat | 1: 500-1: 3000 | brain, colon, kidney, ovary, small intestine |
Background
MYH10 (non-muscle II-b, NM IIB) is a member of non-muscle myosin II which plays fundamental roles in the maintenance of cell morphology, cell adhesion, and migration, as well as cell division. MYH10 is mainly present in nerve cells, megakaryocytes, and other non-muscle cells. It has been reported to mediate centrosome reorientation during cell migration and contribute to ciliogenesis. Overexpression of MYH10 has been observed in breast cancer.
Images
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Western blot analysis of MYH10 (GB111186) at dilution of 1: 500 |
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Immunohistochemistry of paraffin embedded mouse brain using MYH10 (GB111186) at dilution of 1: 1400 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse kidney using MYH10 (GB111186) at dilution of 1: 1400 (400x lens) |
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Immunohistochemistry of paraffin embedded rat brain using MYH10 (GB111186) at dilution of 1: 1400 (400x lens) |
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Immunohistochemistry of paraffin embedded rat kidney using MYH10 (GB111186) at dilution of 1: 1400 (400x lens) |
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Immunohistochemistry of paraffin embedded rat ovary using MYH10 (GB111186) at dilution of 1: 1400 (400x lens) |
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Immunofluorescence of paraffin embedded mouse brain using MYH10 (GB111186) at dilution of 1: 1400 (400x lens) |
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Immunofluorescence of paraffin embedded mouse small intestine using MYH10 (GB111186) at dilution of 1: 1400 (400x lens) |
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Immunofluorescence of paraffin embedded rat brain using MYH10 (GB111186) at dilution of 1: 1400 (400x lens) |
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Immunofluorescence of paraffin embedded rat colon using MYH10 (GB111186) at dilution of 1: 1400 (400x lens) |
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Immunofluorescence of paraffin embedded rat kidney using MYH10 (GB111186) at dilution of 1: 1400 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
