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Anti-MYLK3/MLCK Rabbit pAb (100 μl)
| Reactivity: | M & M |
| Applications: | WB & IHC |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | MYLK3/MLCK rabbit polyclonal |
Gene Name: | Myosin light chain kinase 3 |
Synonyms: | caMLCK, MLCK, MLCK2, MYLK3, myosin light chain kinase 3, Putative myosin light chain kinase 3 |
Immunogen: | Recombinant protein corresponding to Mouse MYLK3/MLCK |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | |
Predicted MW. | 86 kDa |
Observed MW. | 100 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Mouse | 1: 300-1: 800 | heart |
IHC | Mouse | 1: 300-1: 800 | heart |
Background
The Ca2+/calmodulin-dependent protein kinases (CaM kinases) are a structurally related subfamily of serine/threonine kinases that includes CaMKI, CaMKII, CaMKIV and Myosin light chain kinases (MYLKs, also designated MLCKs). The MYLK kinases phosphorylate Myosin regulatory light chains to catalyze Myosin interaction with Actin filaments, resulting in contractile activity. MYLK3 (Myosin light chain kinase 3) is a 795 amino acid cardiac-specific protein that contains one protein kinase domain and belongs to the protein kinase superfamily. Like other MYLK kinases, MYLK3 is thought to play a role in smooth muscle contraction, specifically using magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of Myosin light chain proteins. Additionally, MYLK3 may regulate sarcomere assembly in heart tissue, possibly mediating proper heart function.
Images
| Western blot analysis of MYLK3 (GB113358) at dilution of 1: 800 |
 | Immunohistochemistry analysis of paraffin-embedded mouse heart using MYLK3 (GB113358) at dilution of 1: 1200 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
