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Recombinant Anti-Mast cell tryptase Rabbit mAb (100 μl)
| Reactivity: | H,M |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Monoclonal |
| Gene Name: | Tryptase alpha/beta-1 |
Synonyms: | MCP7, TPS1, TPS2, TPSAB1, TPSB1, Tryptase 1, tryptase alpha/beta 1, Tryptase beta 1, Tryptase I |
Immunogen: | Recombinant protein corresponding to Human Mast cell tryptase |
Uniprot ID: | Q15661 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Secreted |
Product Usage Information
IHC/IF | Human, Mouse | 1: 500-1: 1000 | colon, skin |
Background
Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. May play a role in innate immunity. Isoform 2 cleaves large substrates, such as fibronectin, more efficiently than isoform 1, but seems less efficient toward small substrates.
Images
| IHC analysis of Mast cell tryptase (GB15110). Sample: Human colon, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of Mast cell tryptase (GB15110). Sample: Mouse skin, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
