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Anti-MeCP2 Rabbit pAb (100 μl)
| Reactivity: | H,M & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | MeCP2 rabbit polyclonal antibody |
Gene Name: | Methyl-CpG-binding protein 2 |
Synonyms: | AUTSX3, MeCp 2 protein, MECP2, Methyl CpG binding protein 2, MRX16, MRX79, MRXS13, MRXSL, PPMX, RTS, RTT |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse MeCP2 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. | 52 kDa |
Observed MW. | 72 kDa |
Uniprot ID: | Q9Z2D6, Q00566 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse | 1:500-1:1000 | HeLa, SH-SY5Y, NIH/3T3, Jurkat |
IHC/IF | Mouse, Rat | 1:200-1:600 | brain, kidney, lung, spinal cord, spleen |
Background
MECP2 belongs to a family of nuclear proteins (including MBD1, MBD2, MBD3 and MBD4) that have a methyl-CpG binding domain. MECP2 is capable of binding specifically to methylated DNA. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. MECP2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development.
Images
 | Western blot analysis of Mecp2 (GB111973) at dilution of 1: 500 |
| Immunohistochemistry of paraffin embedded mouse brain using MeCP2 (GB111973) at dilution of 1: 200 (400x lens) |
| Immunohistochemistry of paraffin embedded mouse lung using MeCP2 (GB111973) at dilution of 1: 200 (400x lens) |
| Immunohistochemistry of paraffin embedded mouse spinal cord using MeCP2 (GB111973) at dilution of 1: 200 (400x lens) |
| Immunohistochemistry of paraffin embedded rat brain using MeCP2 (GB111973) at dilution of 1: 200 (400x lens) |
| Immunohistochemistry of paraffin embedded rat lung using MeCP2 (GB111973) at dilution of 1: 200 (400x lens) |
| Immunofluorescence of paraffin embedded mouse brain using MeCP2 (GB111973) at dilution of 1: 200 (400x lens) |
| Immunofluorescence of paraffin embedded mouse spleen using MeCP2 (GB111973) at dilution of 1: 200 (400x lens) |
| Immunofluorescence of paraffin embedded rat kidney using MeCP2 (GB111973) at dilution of 1: 200 (400x lens) |
| Immunofluorescence of paraffin embedded rat lung using MeCP2 (GB111973) at dilution of 1: 200 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
