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| Reactivity: | M,R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Myf5 rabbit polyclonal antibody |
Gene Name: | Myogenic factor 5 |
Synonyms: | Myf-5, Myf5, bHLHc2, Class C basic helix-loop-helix protein 2 |
Immunogen: | Recombinant protein corresponding to Mouse Myf5 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 28 kDa |
Observed MW. | 39 kDa |
Uniprot ID: | P24699 |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Mouse, Rat | 1: 500-1: 1000 | skeletal muscle |
Background
Differentiation of myogenic cells is regulated by multiple positively and negatively acting factors. One well characterized family of helix-loop-helix (HLH) proteins known to play an important role in the regulation of muscle cell development include Myo D, myogenin, Myf-5 and Myf-6 (also designated MRF-4 or herculin). Of interest, most muscle cells express either Myo D or Myf-5 in the committed state, but when induced to differentiate, all turn on expression of myogenin. Myo D transcription factors form heterodimers with products of a more widely expressed family of bHLH genes, the E family, which consists of at least three distinct genes: E2A, IF2 and HEB. Myo D-E heterodimers bind avidly to consensus (CANNTG) E box target sites that are functionally important elements in the upstream regulatory sequences of many muscle-specific terminal differentiation genes.
Images
 | Western blot analysis of Myf5 (GB112330) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
