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Anti-Myosin Light Chain 2 Rabbit pAb (100 μl)
| Reactivity: | H |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Myosin Light Chain 2 rabbit polyclonal antibody |
Gene Name: | Myosin regulatory light chain 2, ventricular/cardiac muscle isoform |
Synonyms: | MYL2, CMH10, MLC2, MLC-2s/v, myosin |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse MYL2 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | A band |
Uniprot ID: | P10916, P51667, P08733 |
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Applications
Applications | Species | Dilution | Positive Sample |
IHC/IF | Mouse, Rat | 1: 200-1: 1000 | heart, Skeletal muscle |
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Background
Contractile protein that plays a role in heart development and function . Following phosphorylation, plays a role in cross-bridge cycling kinetics and cardiac muscle contraction by increasing myosin lever arm stiffness and promoting myosin head diffusion; as a consequence of the increase in maximum contraction force and calcium sensitivity of contraction force. These events altogether slow down myosin kinetics and prolong duty cycle resulting in accumulated myosins being cooperatively recruited to actin binding sites to sustain thin filament activation as a means to fine-tune myofilament calcium sensitivity to force.
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Images
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Immunohistochemistry analysis of paraffin-embedded mouse muscle using MYL2(GB11417) at dilution of 1:800 |
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Immunohistochemistry analysis of paraffin-embedded rat muscle using MYL2(GB11417) at dilution of 1:600 |
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Immunofluorescent analysis of paraformaldehyde-fixed mouse heart using MYL2(GB11417) at dilution of 1:200 |
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Immunofluorescent analysis of paraformaldehyde-fixed rat heart using MYL2(GB11417) at dilution of 1:200 |
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Immunofluorescent analysis of paraformaldehyde-fixed rat skeletal muscle using MYL2(GB11417) at dilution of 1:200 |
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Immunofluorescent analysis of paraformaldehyde-fixed rat muscle using MYL2(GB11417) at dilution of 1:200 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
