Reactivity: | H,M,R |
Applications: | WB |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | N4BP1 rabbit polyclonal |
Gene Name: | NEDD4-binding protein 1 |
Synonyms: | N4BP1, N4bp1, Kiaa0615, FLJ31821, MGC176730 |
Immunogen: | Recombinant protein corresponding to Mouse N4BP1 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 99 kDa |
Observed MW. | 99 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | testis |
Background
Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates .
Images
Western blot analysis of N4BP1 (GB113015) at dilution of 1: 1000 Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: Mouse testis tissue lysate Lane 4: Rat testis tissue lysate |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |