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Anti-NARFL Rabbit pAb (100 μl)
| Reactivity: | H & H |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Cytosolic iron-sulfur assembly component 3 |
Synonyms: | Cytosolic Fe-S cluster assembly factor NARFL, Iron-only hydrogenase-like protein 1, IOP1, Ciao3, uclear prelamin A recognition factor-like, LET1L, PRN, Nuclear prelamin A recognition factor-like protein |
Immunogen: | Recombinant protein corresponding to Mouse NARFL |
Uniprot ID: | Q7TMW6 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 53 kDa / 53 kDa |
Product Usage Information
WB | Human | 1: 500-1: 1000 | HeLa, HEK293, A549 |
ICC/IF | Human | 1: 50-1: 200 | Hela |
Background
NARFL, also named as PRN and IOP1, belongs to the NARF family. NAFL is a component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. It is required for maturation of extramitochondrial Fe/S proteins. It seems to negatively regulate the level of HIF1A expression, although this effect could be indirect. The antibody is specific to NARFL.
Images
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Western blot analysis of NARFL (GB111621) at dilution of 1: 500 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed HeLa cells using NARFL(GB111621) at dilution of 1: 50-1: 200 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |