Your shopping cart is empty!
Anti-NEDD4 Rabbit pAb (100 μl)
| Reactivity: | M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | NEDD4 rabbit polyclonal antibody |
Gene Name: | E3 ubiquitin-protein ligase NEDD4 |
Synonyms: | HECT-type E3 ubiquitin transferase NEDD4, Kiaa0093, Neural precursor cell expressed developmentally down-regulated protein 4, Nedd4, Nedd4-1, Nedd4a |
Immunogen: | Recombinant protein corresponding to Mouse NEDD4 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 103 kDa |
Observed MW. | 120 kDa |
Uniprot ID: | P46935, Q62940 |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Mouse, Rat | 1: 500-1: 1000 | brain, lung, cerebral cortex |
Background
The E3 ubiquitin-protein ligase NEDD4 is a Ubl conjugation pathway protein. It contains one HECT (E6AP-type E3 ubiquitin-protein ligase) domain and four WW domains. It is involved in the pathway leading to the degradation of VEGFR-2/kDFR, independently of its ubiquitin-ligase activity. It acts as a potential proto-oncogene that negatively regulates PTEN via ubiquitination, a paradigm analogous to that of Mdm2 and p53. It is also reported to be involved in the budding of many viruses and is the regulator of epithelial Na+ channels, which lead to arterial hypertension. NEDD4 protein is localized to the sarcolemmal region of muscle fibers. Increase in NEDD4 protein expression leads to unloaded and denervated muscles as well as a decrease in Notch1 expression. Hence it acts as down regulator of Notch-1 gene expression.
Images
|
|
Western blot analysis of nedd4 (GB111057) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
