Anti-Nephrin Rabbit pAb (100 μl)

Anti-Nephrin Rabbit pAb (100 μl)

Brand: ServiceBio
Cat. #: GB115655-100
Availability: In Stock
$259.00
-+
Reactivity: R    
Applications: IHC/IF    
Host Species: Rabbit    
Clonality: Polyclonal    
Gene Name:

Nephrin

Synonyms:

NPHS1, CNF, NPHN, nephrin, NPHS1 nephrin, nephrin

Immunogen:

KLH conjugated Synthetic peptide corresponding to Rat nephrin

Uniprot ID:

Q9R044

Isotype:

IgG

Purity:

Affinity purification

Subcellular location:

Cell membrane

Predicted MW. / Observed MW.

134 kDa / 165-200 kDa




Product Usage Information

IHC/IF

Rat

1: 1000-1: 2000

kidney




Background

Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion.




Images

01 GB115655_Nephrin_C3382_C338223082202_d500_R kidney_IHC-WM.png

IHC analysis of Nephrin(GB115655).
Sample: Rat kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C 20min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

02 GB115655_Nephrin_C3382_C338223082202_d500_R kidney_IHC-WM(7).png

IHC analysis of Nephrin(GB115655).
Sample: Rat kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C 20min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.




Storage

Storage            Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer            PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.


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