Reactivity: | H |
Applications: | WB |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Gene Name: | Nestin |
Synonyms: | NES, Nbla00170, nestin |
Immunogen: | Recombinant protein corresponding to Human Nestin |
Uniprot ID: | P48681 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 177 kDa / 250-300 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
WB | Human | 1: 300-1: 500 | A172, SK-N-SH, U-251 MG |
Background
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.
Images
WB analysis of Nestin(GB115683). |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |