Anti-Nestin Rabbit pAb (100 μl)

Anti-Nestin Rabbit pAb (100 μl)

Brand: ServiceBio
Cat. #: GB115685-100
Availability: In Stock
$259.00
-+
Reactivity: H    
Applications: IHC/IF    
Host Species: Rabbit    
Clonality: Polyclonal    
Gene Name:

Nestin

Synonyms:

NES, Nbla00170, nestin

Immunogen:

KLH conjugated Synthetic peptide corresponding to Human Nestin

Uniprot ID:

P48681

Isotype:

IgG

Purity:

Affinity purification

Subcellular location:

Cytoplasm, intermediate filament cytoskeleton




Product Usage Information

IHC/IF

Human

1: 500-1: 1000

glioblastoma multiforme, kidney




Background

Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells.




Images

01 GB115685_Nestin-C3359-C335923082202_d500_H glioblastoma multiforme_20.0x_IHC_WM.png

IHC analysis of Nestin(GB115685).
Sample: Human glioblastoma multiforme, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C 20min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

02 GB115685_Nestin-C3359-C335923082202_d500_H kidney_20.0x_IHC_WM.png

IHC analysis of Nestin(GB115685).
Sample: Human kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C 20min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.




Storage

StorageStore at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage BufferPBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.


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