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Anti-Nova1 Rabbit pAb (100 μl)
| Reactivity: | M,R & R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | RNA-binding protein Nova-1 |
Synonyms: | Nova-1, Onconeural ventral antigen 1, Ventral neuron-specific protein 1, Neuro-oncological ventral antigen 1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Nova1 |
Uniprot ID: | Q9JKN6 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. / Observed MW. | 52 kDa / 52 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 1000-1: 2000 | brain |
IHC/IF | Rat | 1: 1000-1: 3000 | brain, cerebellum |
Background
Functions to regulate alternative splicing in neurons by binding pre-mRNA in a sequence-specific manner to activate exon inclusion or exclusion . It binds specifically to the sequences 5'-YCAY-3' and regulates splicing in only a subset of regulated exons . Binding to an exonic 5'-YCAY-3' cluster changes the protein complexes assembled on pre-mRNA, blocking U1 snRNP binding and exon inclusion, whereas binding to an intronic 5'-YCAY-3' cluster enhances spliceosome assembly and exon inclusion . Binding to 5'-YCAY-3' clusters results in a local and asymmetric action to regulate spliceosome assembly and alternative splicing in neurons.
Images
| Western blot analysis of NOVA1 (GB114821) at dilution of 1: 1000 |
| Immunohistochemistry of paraffin embedded rat brain using NOVA1 (GB114821) at dilution of 1: 1000 |
| Immunofluorescence of paraffin embedded rat cerebellum2 using NOVA1 (GB114821) at dilution of 1: 1000 |
| Immunofluorescence of paraffin embedded rat cerebellum using NOVA1 (GB114821) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
