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Anti-Nucleolin Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Nucleolin |
Synonyms: | Protein C23, Ncl, Nuc, C23 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Nucleolin |
Uniprot ID: | P09405 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Nucleus |
Predicted MW. / Observed MW. | 77 kDa / 110 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | brain, liver, pancreas |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | breast cancer, prostate, colon |
Background
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
Images
| Western blot analysis of C23 (GB112016) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded human breast cancer using C23 (GB112016) at dilution of 1: 550 |
| Immunofluorescence analysis of paraffin embedded rat colon using C23 (GB112016) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
