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Anti-Osteocalcin Rabbit pAb (100 μl)
| Reactivity: | M,R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Osteocalcin |
Synonyms: | BGLAP, BGP, OC, OCN, Osteocalcin, bone gamma-carboxyglutamate protein |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Osteocalcin |
Uniprot ID: | P86546 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Secreted |
Predicted MW. / Observed MW. | 11 kDa / 11 kDa |
Product Usage Information
IHC/IF | Mouse, Rat | 1: 500-1: 1000 | bone |
Background
Osteocalcin is secreted solely by osteoblasts and thought to play a role in the body's metabolic regulation and is pro-osteoblastic, or bone-building, by nature.It is also implicated in bone mineralization and calcium ion homeostasis. Osteocalcin acts as a hormone in the body, causing beta cells in the pancreas to release more insulin, and at the same time directing fat cells to release the hormone adiponectin, which increases sensitivity to insulin.Osteocalcin acts on Leydig cells of the testis to stimulate testosterone biosynthesis and therefore affectmale fertility.Osteocalcin also acts on myocytes to promote energy availability and utilization and in this manner favors exercise capacity.
Images
| IHC analysis of Osteocalcin(GB115684). Sample: Mouse bone, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 95°C 15min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of Osteocalcin(GB115684). Sample: Rat bone, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 95°C 15min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of Osteocalcin(GB115684). Sample: Mouse bone, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 95°C 15min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of Osteocalcin(GB115684). Sample: Rat bone, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 95°C 15min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
