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| Reactivity: | H,M,R |
| Applications: | IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Tumor protein 63 |
Synonyms: | KET, P63, P73H, P73L, TP73L |
Immunogen: | Recombinant protein corresponding to Human P63 |
Uniprot ID: | Q9H3D4 |
Isotype: | IgG1,k |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. / Observed MW. | 63 kDa /75 kDa /37-85 kDa / 63 kDa /75 kDa /37-85 kDa |
Product Usage Information
IHC/IF | Human, Mouse, Rat | 1: 500-1: 500 | cervical carcinoma,tonsils,bladder |
Background
Acts as a sequence specific DNA binding transcriptional activator or repressor.The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity.Isoform 2 activates RIPK4 transcription.May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes.Involved in Notch signaling by probably inducing JAG1 and JAG2.
Images
| IHC analysis of P63(GB12900). Sample: Human cervical carcinoma, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200, RT, 1h. |
| IHC analysis of P63(GB12900). Sample: Human tonsils, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200, RT, 1h. |
| IHC analysis of P63(GB12900). Sample: Mouse bladder, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200, RT, 1h. |
| IHC analysis of P63(GB12900). Sample: Mouse prostate, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200, RT, 1h. |
| IHC analysis of P63(GB12900). Sample: Mouse skin, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200, RT, 1h. |
| IHC analysis of P63(GB12900). Sample: Rat bladder, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200, RT, 1h. |
| IHC analysis of P63(GB12900). Sample: Rat skin, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |