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| Reactivity: | H,M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | PABP rabbit polyclonal antibody |
Gene Name: | Polyadenylate-binding protein 1 |
Synonyms: | PABP-1, Poly(A)-binding protein 1, Pabpc1, Pabp1 |
Immunogen: | Recombinant protein corresponding to Mouse PABP |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Cytoplasm |
Predicted MW. | 71 kDa |
Observed MW. | 71 kDa |
Uniprot ID: | P29341, Q9EPH8 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | liver, testis, brain |
IHC/IF | Mouse, Rat | 1: 1000-1: 4000 | testis |
Background
PABPC1 binds the poly(A) tail of mRNA. It may be involved in cytoplasmic regulatory processes of mRNA metabolism such as pre-mRNA splicing. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. PABPC1 can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. It may be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.
Images
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Western blot analysis of pabp1 (GB111164) at dilution of 1: 500 |
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Immunohistochemistry of paraffin embedded mouse testis using pabp1 (GB111164) at dilution of 1: 1200 (400x lens) |
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Immunohistochemistry of paraffin embedded rat testis using pabp1 (GB111164) at dilution of 1: 1200 (400x lens) |
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Immunofluorescence of paraffin embedded mouse testis using pabp1 (GB111164) at dilution of 1: 3913 (400x lens) |
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Immunofluorescence of paraffin embedded rat testis using pabp1 (GB111164) at dilution of 1: 3913 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
