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| Reactivity: | H,M & H |
| Applications: | WB & IHC |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | PARN rabbit polyclonal |
Gene Name: | Poly(A)-specific ribonuclease PARN |
Synonyms: | DAN, Deadenylating nuclease, Deadenylation nuclease, PARN |
Immunogen: | Recombinant protein corresponding to Mouse PARN |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | |
Predicted MW. | 72 kDa |
Observed MW. | 76 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse | 1: 500-1: 1000 | brain |
IHC | Human | 1: 900-1: 1800 | colon cancer, stomach |
Background
The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Images
| Western blot analysis of PARN (GB113443) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded human colon cancer using PARN (GB113443) at dilution of 1: 1800 |
| Immunohistochemistry analysis of paraffin-embedded human stomach using PARN (GB113443) at dilution of 1: 1800 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
