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Anti-PARP1 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | PARP1 rabbit polyclonal antibody |
Gene Name: | Poly [ADP-ribose] polymerase 1 |
Synonyms: | PARP 1, PARP-1, PARP1, ADPRT, ADPRT1, msPARP, PPOL, ARTD1, PARP, PARP-1, pADPRT-1, oly (ADP ribose) polymerase 1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human PARP1 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. | 113 kDa |
Observed MW. | 116 kDa |
Uniprot ID: | P09874, P11103, P27008 |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | HEK293, Jurkat, MCF7 |
IHC/IF | Human, Mouse, Rat | 1: 700-1: 1600 | brain, colon cancer, heart |
Background
PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis.
Images
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WB analysis of PARP (GB111500). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
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IHC analysis of PARP (GB111500). Sample: Human colon cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 800, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
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IHC analysis of PARP (GB111500). Sample: Mouse brain (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 800, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
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IHC analysis of PARP (GB111500). Sample: Mouse kidney (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 800, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
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IHC analysis of PARP (GB111500). Sample: Rat kidney (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 800, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
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IHC analysis of PARP (GB111500). Sample: Rat testis (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 800, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
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IF analysis of PARP (GB111500). Sample: Rat heart (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98°C,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 700, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
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IF analysis of PARP (GB111500). Sample: Rat testis (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98°C,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 700, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
