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| Reactivity: | M & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Pericentriolar material 1 protein |
Synonyms: | hPCM 1, PCM 1, PCM1, pericentriolar material 1, PTC4 |
Immunogen: | Recombinant protein corresponding to Mouse PCM1 |
Uniprot ID: | Q9R0L6 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Cytoskeleton |
Predicted MW. / Observed MW. | 229 kDa / 260 kDa |
Product Usage Information
WB | Mouse | 1: 800-1: 1200 | testis |
IHC/IF | Mouse, Rat | 1: 600-1: 1200 | heart |
Background
Required for centrosome assembly and function (PubMed:12112146). Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2 (By similarity). Required to anchor microtubules to the centrosome (By similarity). Involved in the biogenesis of cilia (By similarity).
Images
| Western blot analysis of Pcm1 (GB114537) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded mouse heart using Pcm1 (GB114537) at dilution of 1: 1200 |
| Immunohistochemistry analysis of paraffin embedded rat heart using Pcm1 (GB114537) at dilution of 1: 1200 |
| Immunofluorescence analysis of paraffin embedded mouse heart using Pcm1 (GB114537) at dilution of 1: 600 |
| Immunofluorescence analysis of paraffin embedded rat heart using Pcm1 (GB114537) at dilution of 1: 600 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
