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| Reactivity: | H,M,R & H,M,R & H,M,R & H,M,R |
| Applications: | WB & IHC/IF & ICC/IF & IP |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Proliferating cell nuclear antigen |
Synonyms: | PCNA, ATLD2, proliferating cell nuclear antigen |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse PCNA |
Uniprot ID: | P17918 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. / Observed MW. | 29 kDa / 36 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | HeLa, SW480, B16 |
IHC/IF | Human, Mouse, Rat | 1: 200-1: 1000 | intestinal cancer, liver cancer, lung cancer |
ICC/IF | Human, Mouse, Rat | 1:200-1: 400 | HeLa |
IP | Human, Mouse, Rat | 1: 50-1: 200 | HeLa cells |
Background
Proliferating cell nuclear antigen (PCNA) is a DNA clamp that acts as a processivity factor for DNA polymerase δ in eukaryotic cells and is essential for replication. PCNA is a homotrimer and achieves its processivity by encircling the DNA, where it acts as a scaffold to recruit proteins involved in DNA replication, DNA repair, chromatin remodeling and epigenetics.
Images
| Western blot analysis of PCNA (GB11010) at dilution of 1: 1000 |
| Western blot analysis of PCNA (GB11010) at dilution of 1: 1000 |
| Immunohistochemistry of paraffin embedded human liver cancer using PCNA (GB11010) at dilution of 1: 500 (200x lens) |
| Immunohistochemistry of paraffin embedded human lung cancer using PCNA (GB11010) at dilution of 1: 500 (200x lens) |
| Immunofluorescence of paraffin embedded human intestinal cancer using PCNA (GB11010) at dilution of 1: 500 (200x lens) |
| Immunofluorescence of paraffin embedded human intestinal cancer using PCNA (GB11010) at dilution of 1: 500 (200x lens) |
| Immunofluorescence of paraffin embedded rat testis using PCNA (GB11010) at dilution of 1: 500 (200x lens) |
| Immunofluorescence of paraffin embedded mouse testis using PCNA (GB11010) at dilution of 1: 500 (200x lens) |
| Immunoprecipitation of HeLa cells lysate using PCNA（IP: GB11010, 1: 100; Detection: GB12010, 1: 2000） |
| ICC analysis of PCNA(GB11010)red. Sample: HeLa, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 300, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
