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| Reactivity: | H,M,R & H,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | PDCD4 rabbit polyclonal antibody |
Gene Name: | Programmed cell death protein 4 |
Synonyms: | Topoisomerase-inhibitor suppressed protein, Death up-regulated gene protein, MGC33046, MGC33047, Protein MA-3, Ma3, Tis, Dug, H731, Neoplastic transformation inhibitor, Nuclear antigen H731, Protein 197/15a, RP11 348N5.4, Nuclear antigen H731-like |
Immunogen: | Recombinant protein corresponding to Mouse PDCD4 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Cytoplasm |
Predicted MW. | 52 kDa |
Observed MW. | 64 kDa |
Uniprot ID: | Q53EL6, Q61823, Q9JID1 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | spleen, thymus, colon |
IHC/IF | Human, Rat | 1: 400-1: 1600 | breast cancer, colon cancer, spleen, thymus |
Background
The Pdcd4 gene encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells.Two transcripts encoding different isoforms have been identified. Programmed cell death 4 (Pdcd4) is a novel tumor suppressor that inhibits translation, progression and invasion. It was first identified as being differnetially upregulated during apoptosis. Pdcd4 interferes with the activity of the eukaryotic initiation factor (eIF) 4A by displacing the scaffold protein eIF4G from its binding to the RNA helicase eIF4A.
Images
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Western blot analysis of pdcd4 (GB11991) at dilution of 1: 500 |
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Immunohistochemistry of paraffin embedded human breast cancer using pdcd4 (GB11991) at dilution of 1: 700 (200x lens) |
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Immunohistochemistry of paraffin embedded human colon cancer using pdcd4 (GB11991) at dilution of 1: 700 (250x lens) |
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Immunohistochemistry of paraffin embedded rat spleen using pdcd4 (GB11991) at dilution of 1: 700 (250x lens) |
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Immunohistochemistry of paraffin embedded rat thymus using pdcd4 (GB11991) at dilution of 1: 700 (250x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
