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Anti-PDHA1 Rabbit pAb (100 μl)
| Reactivity: | M,R & H |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial |
Synonyms: | PDHE1-A type I, Pdha1, Pdha-1, PHE1A, PDHCE1A |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse PDHA1 |
Uniprot ID: | P35486 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 43 kDa / 43 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 350-1: 700 | heart, liver, brain |
ICC/IF | Human | 1: 100-1: 400 | Hela |
Background
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase(E3).
Images
| Western blot analysis of PDHA1 (GB112509) at dilution of 1: 350 |
| Western blot analysis of PDHA1 (GB112509) at dilution of 1: 350 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed HeLa cells using PDHA1(GB112509) at dilution of 1: 100-1: 400 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
