Reactivity: | H,M,R |
Applications: | WB |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | PHKG2 rabbit polyclonal |
Gene Name: | Phosphorylase b kinase gamma catalytic chain, liver/testis isoform |
Synonyms: | GSD9C, PHK gamma T, PHKG2, PHK-gamma-LT, Phosphorylase kinase subunit gamma-2, PSK C3 |
Immunogen: | Recombinant protein corresponding to Mouse PHKG2 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 47 kDa |
Observed MW. | 47 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | liver, colon, testis |
Background
Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, and encoded by one gene. The gamma subunit also includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration.
Images
Western blot analysis of PHKG2 (GB113538) at dilution of 1: 2000 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |