Reactivity: | M,R |
Applications: | WB |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Gene Name: | Phosphatidylinositol 3-kinase regulatory subunit alpha |
Synonyms: | PIK3R1, AGM7, GRB1, IMD36, P85, p85-ALPHA, Phosphoinositide-3-kinase regulatory subunit 1, PI 3 Kinase p85 alpha |
Immunogen: | Recombinant protein corresponding to Human PI 3 Kinase p85 alpha |
Uniprot ID: | P27986 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 85 kDa / 85 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | heart, thymus, liver |
Background
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. PIK3R1 encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance.
Images
Western blot analysis of PIK3R1 (GB11525) at dilution of 1: 500 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |